Technology

We make bioprocess analytics faster and smarter.
Maintaining Critical Quality Attributes (CQAs) is fundamental to ensuring the safety, efficacy, and consistency in biopharmaceutical production. In downstream processing, Monipa accurately measures protein and excipient concentrations, enabling control over attributes that can potentially impact the final product. By leveraging mid-infrared (MIR) polarization stabilized spectroscopy, we provide you with a robust, direct, and label-free analysis of concentration levels across a wide range, e.g.  0.5–250 mg/mL. Moreover, Monipa’s single-use capabilities eliminates the risk of cross-contamination, preventing batch failures and enhancing overall process reliability.

Parallel measurement of CQAs by mid-infrared spectroscopy

High robustness through polarization stabilized spectroscopy.
Spectroscopy allows for real-time, simultaneous monitoring of multiple parameters in bioprocess applications, typically covering wavelengths from ultraviolet (UV) to mid-infrared. Infrared spectroscopy is categorized into three regions: far-infrared, mid-infrared, and near-infrared. Our technology focuses on mid-infrared spectroscopy, offering non-destructive, non-intrusive and precise analytical capabilities. 

The fundamental principles of spectroscopy are rooted in molecular absorption, where molecules absorb energy at distinct wavelengths. The quantitative analysis confirms the proportionality between absorption intensity and concentration as well as the relationship between the unique absorption profiles and differentiation of molecular structures. This direct method provides measurements without intermediaries, allowing for enhanced control over dynamic bioprocesses.

Our speciality, polarization stabilized spectroscopy, enables background correction by using background measurements that correct the spectra for changes in the instrument or the environment. As a result, Monipa evades problems with absorption from water vapor.

Reduced contamination risk with

Single-use flow cell

In infrared spectroscopy there are different measurement methods such as Transmission, Reflection, and Attenuated Total Reflection (ATR). Our Process Analytical Technology solution, Monipa, works with ATR spectroscopy, which offers the following advantages over other methods:

An ATR crystal is a key component in infrared spectroscopy and the refractive index is an essential factor in selecting an ATR crystal. Only materials with a high refractive index, like diamond or zinc selenide, can be used. However, these materials are costly and not ideal for single-use applications, such as bioprocess monitoring. IRUBIS’ ATR crystal is based on silicon, a widely available material used in semiconductor manufacturing. It enables the production of large quantities of ATR crystals, resulting in lower costs. This makes our ATR crystals an ideal choice for single-use applications.