MONIPA
About Monipa
Monipa focuses on simplified bioprocess monitoring: our measurement system is based on mid-infrared (MIR) spectroscopy and monitors multiple bioprocesses inline at the same time. Spectroscopy is a powerful technique that allows the analysis of various relevent parameters. Therefore Monipa is beneficial in both upstream bioprocessing; in metabolite monitoring and control, as well as in downstream bioprocessing to aid in contaminant detection or monitoring of target proteins and excipients.
Our Single-Use Flow Cell
The single-use flow cell is one of the core elements of Monipa. An easy screw mechanism enables a stable fixation of the flow cell. At the same time it allows the replacement of the flow cell after each monitored process, reducing contamination risk. The flow cell contains our single-use silicon ATR crystal, enabling a disposable unit. By connecting the flow cell via loop to e.g. the bioreactor or a chromatography column, different parameters can be monitored online or inline. Stable measurement conditions are achieved due to the external flow cell, that is mounted e.g. outside of the bioreactor. This prevents disturbances of the measurements by gas bubbles or by the stirrer which both can occur inside the bioreactor.
MONIPA OVERVIEW
No Calibration Model Building
Calibration model building is an important step for spectroscopic devices. The spectra depends on the molecules in the measured samples. Following this, also different cell lines, cell culture media, metabolite or nutrient concentration leads to changes in the spectra. Calibration models are built by correlating the spectroscopic measurements to the reference method.
IRUBIS’ inline and online monitoring solution Monipa offers an innovative approach to solve the time extensive and costly calibration model building. IRUBIS one point calibration is based on a relative measurement. Only the known start-value of the parameter need to be included. Consequently the measurement of reference processes for calibration model building are not necessary.
Monitoring and Control in Bioreactors
The use of MIR spectroscopy for inline measurement of glucose and lactate in mammalian cell cultures has already been demonstrated in recent studies (Sandor et al., 2013; Wu et al., 2015). To point out, drawbacks are high equipment costs and low robustness of the ATR probes. IRUBIS has managed to solve many of these teething issues with our Monipa system.
Benefits in Upstream Processing
- 24/7 inline monitoring of metabolites and nutrients as well as glucose control
- Real-time data and glucose control (closed loop)
- Single-use flow cell decreases contamination risk
- Parallel monitoring of multiple parameters
- Increased robustness compared to other spectroscopic methods
- Reduced calibration time by one-point calibration
- Suitable to to a variety of different cell types; from CHO mammalian cells, to microorganisms such as bacteria
Integration into bioreactors
Monipa is compatible with different kind of bioreactors and processes. It can be integrated inline into the harvest stream in perfusion processes. In addition it is suitable as online monitoring system in fed-batch processes. In another case, the technology has been used in fixed-bed bioreactors at biopharma companies. If you interested how to integrate Monipa into your specific process or in a version to monitor multiple processes in parallel, please leave us a message.
MONIPA IN PERFUSION PROCESSES
MONIPA IN FED-BATCH PROCESSES
Inline Monitoring in Downstream Processing
Monipa adresses a variety of different applications in downstream processing, especially taking into account its fast acquisition time of less than 1 minute per data point. Such applications include protein and excipient concentration monitoring.
Monipa in Ultrafiltration and Diafiltration
In this case, the publication shows the inline measurement of the concentration of excipients and proteins comparing mid-infrared spectroscopy compared to an OD280 assay. The publication shows accuarcy of the measurement system with a calculated coefficient of determination of R² = 0.995.
Milewska et al. Eng Life Sci. 2023;23:e2200050
Protein Secondary Structure
Mid-infrared spectroscopy enables differentiation of protein secondary structure such as alpha helices, beta sheets and random coil structures. In particular, a distinct feature of protein aggregation is the increase of crossed β-sheet structures. Hence, IR Spectroscopy is a useful tool to analyze secondary structures of proteins, and their aggregation in complex samples.
Wang et al (2015), Anal Bioanal Chem 407: 4015-21
Ángela I. López-Lorente , I, A & Mizaikoff, B (2016), Anal Bioanal Chem 408: 2875–89
Partner with Monipa: Improve your Bioprocess
Join Our Newsletter!
Our newsletters are written and curated by our marketing team and application scientists. Firstly, you will receive no hidden advertising or third-party mailing. Secondly, you will only get useful insights about Monipa, updates about new application notes or information about where you can meet us in the near future! In short, upgrade you inbox and get our IRUBIS newsletter every other month!
About Monipa
Monipa focuses on simplified bioprocess monitoring: our measurement system is based on mid-infrared (MIR) spectroscopy and monitors multiple bioprocesses inline at the same time. Spectroscopy is a powerful technique that allows the analysis of various relevent parameters. Therefore Monipa is beneficial in both upstream bioprocessing; in metabolite monitoring and control, as well as in downstream bioprocessing to aid in contaminant detection or monitoring of target proteins and excipients.
Our Single-Use Flow Cell
The single-use flow cell is one of the core elements of Monipa. An easy screw mechanism enables a stable fixation of the flow cell. At the same time it allows the replacement of the flow cell after each monitored process, reducing contamination risk. The flow cell contains our single-use silicon ATR crystal, enabling a disposable unit. By connecting the flow cell via loop to e.g. the bioreactor or a chromatography column, different parameters can be monitored online or inline. Stable measurement conditions are achieved due to the external flow cell, that is mounted e.g. outside of the bioreactor. This prevents disturbances of the measurements by gas bubbles or by the stirrer which both can occur inside the bioreactor.
No Calibration Model Building
Calibration model building is an important step for spectroscopic devices. The spectra depends on the molecules in the measured samples. Following this, also different cell lines, cell culture media, metabolite or nutrient concentration leads to changes in the spectra. Calibration models are built by correlating the spectroscopic measurements to the reference method.
IRUBIS’ inline and online monitoring solution Monipa offers an innovative approach to solve the time extensive and costly calibration model building. IRUBIS one point calibration is based on a relative measurement. Only the known start-value of the parameter need to be included. Consequently the measurement of reference processes for calibration model building are not necessary.
Monitoring and Control in Bioreactors
The use of MIR spectroscopy for inline measurement of glucose and lactate in mammalian cell cultures has already been demonstrated in recent studies (Sandor et al., 2013; Wu et al., 2015). To point out, drawbacks are high equipment costs and low robustness of the ATR probes. IRUBIS has managed to solve many of these teething issues with our Monipa system.
Benefits in Upstream Processing
- 24/7 inline monitoring of metabolites and nutrients as well as glucose control
- Real-time data and glucose control (closed loop)
- Single-use flow cell decreases contamination risk
- Parallel monitoring of multiple parameters
- Increased robustness compared to other spectroscopic methods
- Reduced calibration time by one-point calibration
- Suitable to to a variety of different cell types; from CHO mammalian cells, to microorganisms such as bacteria
Integration into bioreactors
Monipa is compatible with different kind of bioreactors and processes. It can be integrated inline into the harvest stream in perfusion processes. In addition it is suitable as online monitoring system in fed-batch processes. In another case, the technology has been used in fixed-bed bioreactors at biopharma companies. If you interested how to integrate Monipa into your specific process or in a version to monitor multiple processes in parallel, please leave us a message.
MONIPA IN PERFUSION PROCESS
MONIPA IN FED-BATCH PROCESSES
Inline Monitoring in Downstream Processing
Monipa adresses a variety of different applications in downstream processing, especially taking into account its fast acquisition time of less than 1 minute per data point. Such applications include protein and excipient concentration monitoring.
Benefits in Downstream Processing
Monipa in Ultrafiltration and Diafiltration
In this case, the publication shows the inline measurement of the concentration of excipients and proteins comparing mid-infrared spectroscopy compared to an OD280 assay. The publication shows accuarcy of the measurement system with a calculated coefficient of determination of R² = 0.995.
Milewska et al. Eng Life Sci. 2023;23:e2200050
How does Monipa work in Downstream Processing?
Partner with Monipa: Improve your Bioprocess
Join Our Newsletter!
Our newsletters are written and curated by our marketing team and application scientists. Firstly, you will receive no hidden advertising or third-party mailing. Secondly, you will only get useful insights about Monipa, updates about new application notes or information about where you can meet us in the near future! In short, upgrade you inbox and get our IRUBIS newsletter every other month!
