Inline Monitoring in mAb Downstream Processing
Overcoming Challenges in mAb
Process Development
Why inline downstream monitoring matters?
Integration of Monipa into mAb Downstream Processing

Inline UF/DF monitoring with Monipa

UF/DF is one of the most effective separation techniques and a critical step in downstream processing for precisely achieving the target product composition. The process relies on a permeable membrane to selectively separate components as a specific volume of formulation buffer is exchanged. It can be divided into key stages:
Ultrafiltration 1
Accurate timing of UF1 improves consistency and reduces process variability.
Diafiltration
Moving beyond fixed DF volumes with real-time monitoring optimizes buffer usage.
Ultrafiltration 2
Preventing deviations at this stage helps avoid reprocessing or, in the worst case, batch loss.
Ideal UF/DF Scenario

Related assets
Replacing offline process control with instant inline data offered by Process Analytical Technology ensures tight control over UF/DF. Monipa is successfully used to determine protein and various excipient concentrations with inline measurements during UF/DF in mAb downstream processing. Get in touch with us to explore tailored applications to your processes!

Engineering in Life Sciences (2023)
Automized inline monitoring in perfused mammalian cell culture by MIR spectroscopy without calibration model building
Authors: Hannah Marienberg, Nicole Desch, Vitalii Mozin, Lorenz Sykore-Mirle, Anja Müller, Andreas Roth, Mathias Käfer, Rüdiger Neef

Application Note (2023)
Real-time infrared monitoring of an ultra-/diafiltration process
Authors: Adrianna Milewska, Clemens Minnich, Simon Kern
