The tether compound/backfiller ratio impacts protein pore formation in the artificial lipid membrane

The tether that anchors the lipid bilayer to the solid surface is a key component of tethered lipid membranes, which mimic biological membranes. The obtained lipid bilayers with high electrical sealing properties facilitate the application of electrochemical impedance spectroscopy (EIS) to assess conductivity and defectiveness resulting, for example, from pore-forming toxins.