We enable this method by integrating a silicon ATR crystal into a disposable flow-through cell. The spectrometer unit is reusable.
Online monitoring is possible by connecting the system to the bioreactor with a loop (fed-batch) or integrating it into the perfusion stream. This eliminates the need for expensive and sensitive IR fiber optics including expensive ATR diamond prisms.
At the same time the robustness increases and the use in single use bioprocessing becomes possible.