Online Monitoring of Lactate and Glucose using Mid Infrared Spectroscopy
We make bioreactor monitoring easier by introducing our cost-effective measurement system based on mid-infrared (MIR) spectroscopy. It allows real-time glucose and lactate measurement as well as a glucose control. Monitoring of further parameters such as glutamine and ammonium are planned.
Your Benefits
Monipa enables the use of Infrared Spectroscopy in bioprocessing leading to high product gain and product quality
- 24/7 online monitoring of glucose and lactate as well as glucose regulation (closed loop)
- Single use flow cell decreases contamination risk
- Parallel monitoring of up to 4 bioreactors
- Increased robustness compared to other spectroscopic methods
- Reduced calibration time through offline calibration
Current State
The use of mid-infrared (MIR) spectroscopy for online measurement of glucose and lactate in mammalian cell cultures has already been demonstrated in recent studies. (Sandor et al., 2013; Wu et al., 2015) Drawbacks are high equipment costs and low robustness of the ATR probes.
Our Innovation
IRUBIS enables translating this technology into bioprocessing by introducing Monipa, a MIR spectrometer combined with an innovative single use flow cell. The flow cell contains our silicon ATR crystal, making it suitable as a disposable unit.
By connecting the flow cell with a loop (fed-batch) to the bioreactor or integrating it in the perfusion stream cell culture condition can be monitored online without sampling. Additionally, the external flow-cell leads to higher robustness.
